| 產品編號 | bs-1807R |
| 英文名稱 | GPR78 Rabbit pAb |
| 中文名稱 | G蛋白偶聯(lián)受體78抗體 |
| 別 名 | GPCR GPR78; G protein-coupled receptor 78; G protein coupled receptor GPR78; G-protein coupled receptor 78; GPR78; GPR78_HUMAN; PRO19818; UNQ5925. |
| 研究領域 | 免疫學 信號轉導 生長因子和激素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-2000,Flow-Cyt=1μg /test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 39 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPR78: 201-300/363 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. Function: Orphan receptor. Displays a significant level of constitutive activity. Its effect is mediated by G(s)-alpha protein that stimulate adenylate cyclase, resulting in an elevation of intracellular cAMP. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: High level of expression in placenta. Expressed throughout the brain at low level. No expression detected in skeletal muscle, lung, heart, liver, pancreas, or kidney. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q96P69 Gene ID: 27201 Database links: Entrez Gene: 27201 Human Omim: 606921 Human SwissProt: Q96P69 Human Unigene: 350588 Human Unigene: 740355 Human |
| 產品圖片 |
Sample:
A549 Cell (Human) Lysate at 30 ug
Primary: Anti-GPR78(bs-1807R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD
Blank control (blue line): U937 (blue).
Primary Antibody (green line): Rabbit Anti-GPR7 antibody (bs-1807R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 2% paraformaldehyde for 10 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: 293T Cells(blue). Primary Antibody: Rabbit Anti-GPR78/AF488 Conjugated antibody (bs-1807R-AF488), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (bs-1807R-AF488, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
