| 產(chǎn)品編號 | bs-8035R |
| 英文名稱 | GABARAPL1 Rabbit pAb |
| 中文名稱 | γ氨基丁酸受體相關蛋白樣1抗體 |
| 別 名 | APG8 like; APG8L; ATG8; ATG8L; Early estrogen regulated protein; GABA; GABA(A) receptor associated protein like 1; GABARAPL1; gamma aminobutyric acid receptor associated protein like 1; GEC 1; GEC1; Glandular epithelial cell protein 1; GBRL1_HUMAN. |
| 研究領域 | 神經(jīng)生物學 通道蛋白 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Pig,Cow,Dog) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 14 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GABARAPL1: 41-117/117 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
GABARAPL1 belongs to the MAP1 LC3 family. It increases cell surface expression of kappa type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Subunit: Interacts with GABRG2 and beta-tubulin. Interacts with OPRK1. Subcellular Location: Cytoplasm, cytoskeleton. Membrane. Endoplasmic reticulum. Golgi apparatus. Tissue Specificity: Ubiquitous. Expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle. Expressed at very low levels in thymus and small intestine. Similarity: Belongs to the MAP1 LC3 family. SWISS: Q9H0R8 Gene ID: 23710 Database links: Entrez Gene: 100135524 Guinea pig Entrez Gene: 23710 Human Entrez Gene: 57436 Mouse Omim: 607420 Human SwissProt: P60518 Guinea pig SwissProt: Q9H0R8 Human SwissProt: Q8R3R8 Mouse Unigene: 524250 Human Unigene: 14638 Mouse Unigene: 118264 Rat |
| 產(chǎn)品圖片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GABARAPL1 Polyclonal Antibody, Unconjugated(bs-8035R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
